On September 30th , our lab has published a new calibration method of RNA-modification mapping in Nature Methods, entitled “Systematic calibration of epitranscriptomic maps using a synthetic modification-free RNA library”. Zhang Zhang and Tao Chen are the co-first authors. Hong-Xuan Chen, Ying-Yuan Xie, Li-Qian Chen, Yu-Li Zhao, Biao-Di Liu, Dong-Zhao Ma, Guo-Shi Chai, Ying Zhang, Wen-Shuo Zhao has also made contribution to this work. Congratulations!

Recent years have witnessed rapid progress in the field of epitranscriptomics. Functional interpretation of the epitranscriptome relies on sequencing technologies that determine the location and stoichiometry of various RNA modifications. However, contradictory results have been reported among studies, bringing the biological impacts of certain RNA modifications into doubt. Here, we develop a synthetic RNA library resembling the endogenous transcriptome but without any RNA modification. By incorporating this modification-free RNA library into established mapping techniques as a negative control, we reveal abundant false positives resulting from sequence bias or RNA structure. After calibration, precise and quantitative mapping expands the understanding of two representative modification types, N6-methyladenosine (m6A) and 5-methylcytosine (m5C). We propose that this approach provides a systematic solution for the calibration of various RNA-modification mappings and holds great promise in epitranscriptomic studies.

By Yelin Lan